Expression of v-src and chicken c-src in rat cells demonstrates qualitative differences between pp60v-src and pp60c-src
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چکیده
منابع مشابه
Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules
Antiserum to the Rous sarcoma virus (RSV)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed in Escherichia coli. alpha p60 serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (TBR) sera. When RSV-transformed cell lysates were preadsorbed with TBR serum, the remaining lysate contained additional pp60v-src, which was recognized ...
متن کاملImmunocytochemical localization of the neuron-specific form of the c-src gene product, pp60c-src(+), in rat brain.
Neurons express high levels of a variant form of the c-src gene product, denoted pp60c-src(+), which contains a 6 amino acid insert in the amino-terminal half of the c-src protein. We have determined the localization of pp60c-src(+) in neurons using an affinity-purified anti-peptide antibody, referred to as affi-SB12, that exclusively recognizes this neuron-specific form of the c-src gene produ...
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Colorectal cancer as the second leading cause of cancer mortality in the Western Societies represents the third most common type of cancer in all over the world (Midgley & Kerr, 1999; Greenlee et al., 2000; Siegel et al., 2012). In spite of advances in the current colorectal cancer therapies; such as surgical resection, chemotherapy and radiotherapy, the mortality rate is still remained high wi...
متن کاملImmunolocalization of the cellular src protein in interphase and mitotic NIH c-src overexpresser cells
The mouse mAb, mAb 327, that recognizes specifically both pp60v-src and pp60c-src in a wide variety of cells, has been used to determine precisely the various locations of pp60c-src in NIH c-src overexpresser cells, using the technique of immunofluorescence microscopy. In interphase cells, the protein exhibits two main distributions: one that appears uniform and in association with the cell sur...
متن کاملSrc promotes destruction of c-Cbl: implications for oncogenic synergy between Src and growth factor receptors.
Cellular Src and epidermal growth factor receptor (EGFR) collaborate in the progression of certain human malignancies, and their cooverexpression characterizes relatively aggressive animal tumors. Our study addressed the mode of oncogenic cooperation and reports that overexpression of c-Src in model cellular systems results in the accumulation of EGFR at the cell surface. The underlying mechani...
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ژورنال
عنوان ژورنال: Cell
سال: 1984
ISSN: 0092-8674
DOI: 10.1016/0092-8674(84)90308-8